Journal: Molecular metabolism

Article Title: Adipose knockout of H-ferritin improves energy metabolism in mice.

doi: 10.1016/j.molmet.2024.101871

Figure Lengend Snippet: Figure 5: Fth AKO mice exhibited an altered adipose gene expression profile. (A,B) qRT-PCR analysis of Pparg, C/ebpa, Retn, Plin1, AdipoQ, Glut4, Scd1, Lep, Fabp4, Hsl, Atgl, and Mgl expression in iBAT and iWAT of Fth AKO and Fthfl/flmice (n ¼ 3e4, from two independent experiments). (C) Serum leptin of Fth AKO and Fthfl/flmice (n ¼ 3e4). (D) qRT-PCR analysis of Cpt1a and Cpt1b expression in iBAT and iWAT of Fth AKO and Fthfl/flmice (n ¼ 5e6, from two independent experiments). Data was analyzed by two-tailed unpaired t- tests. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Serum leptin Serum leptin concentration was measured by a mouse leptin ELISA kit (ProteinTech, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Two Tailed Test

Journal: Molecular Metabolism

Article Title: Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop

doi: 10.1016/j.molmet.2019.09.008

Figure Lengend Snippet: Hypothalamic leptin receptor knockdown attenuates the adipose redistribution-associated with unilateral iWAT injection of Rec2-Cre in male PTEN flox mice. (A) Experimental design. (B) Hypothalamic Lepr expression. (C) iWAT weight at sacrifice. (D) Ratio of Rec2-treated iWAT to the untreated contralateral iWAT. (E) Relative mRNA expression of leptin in iWAT. (F) Ratio of leptin mRNA between Rec2-treated iWAT and untreated contralateral iWAT. Data are means ± SEM. n = 6–7 per group for body weight and tissue weight data. n = 4–6 for qPCR data. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: To select the most effective microRNA, the miR-Lepr expressing plasmids were co-transfected with a LepR expression plasmid (Lepr, MR226855, ORIGENE) to HEK293T cells.

Techniques: Injection, Expressing